Its long lasting signals allow for multiple exposures. Cytiva Amersham ECL start Western Blotting Detection Reagent Reagent is luminol-based chemiluminescent substrate designed for the detection of proteins immobilized to a membrane Manufacturer: Cytiva RPN3243 View more versions of this product Catalog No. 3. This means that medium to very low protein amounts (low picogram levels) can be detected using highly diluted antibodies. The ECL Advance reagents are sensitive to prolonged exposure to light. Sign In Register. The ECL Western Blot Detection Reagents are sold as a two component kit containing a bottle of luminol substrate and a bottle of peroxide solution. Amersham ECL Select is the most sensitive chemiluminescent Western blotting detection reagent in the Amersham ECL range For longer storage, the pre-mixed solution should be kept at 2-8C, but . Item Amersham ECL Select Western blotting detection reagent; Company Cytiva Life Sciences; Catalog Number RPN2235; . ECL Advance Solution B Proprietary substrate in Tris Key product features: Outstanding signal intensity suitable for the most demanding Western blotting applications, including the detection of low abundance proteins Detects the smallest change in . Description. RPN2236 Solution A: Luminol solution, 3 50 ml Solution B: Peroxide solution, 3 50 ml sufficient for 3000 cm 2 membrane Analysis Note To view the Certificate of Analysis for this product, please visit www.cytiva.com. Amersham ECL Prime reagent addition and at time points up to 3 h post reagent. High signal intensity makes it suitable for the most demanding Western blotting applications, allowing for detection of minute protein quantities. Specifications. RPN2236 GE Healthcare Amersham ECL Prime Western Blotting Detection Reagent Amersham ECL Prime Western Blotting Detection Reagent Each of 1 Rating Required Select Rating 1 star (worst) 2 stars 3 stars (average) 4 stars 5 stars (best) Cytiva - RPN2232 - Amersham ECL Prime Western Blotting Detection Reagent. quantitative analysis using fluorescence Western blotting Probing and detection Amersham ECL Western blotting detection reagents - a variety of reagents, with the best choice depending on the aim of the experiment. Provides convenient handling time between detection and completion of experiment. Amersham ECL Prime Blocking Reagent is blocking Amersham Protran NC and Amersham Hybond PVDF membranes used with Amersham ECL start, Amersham ECL, Amersham, ECL Prime, Amersham ECL Select and Amersham ECL Plex.Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. 45-001-160 $485.00 / Each Quantity Add to cart Description Specifications Amersham ECL Select typically exhibits a dynamic range that is complementary to Amersham ECL Prime, and similar to Amersham ECL Advance. Bio-Rad Clarity Max, Amersham ECL Select, Millipore Immobilon Western, Bio-Rad Clarity and ECL are shown in bar graph form. A schematic representation of the role of Amersham ECL Select in a typical Western blotting procedure. Description. Amersham ECL Select detection reagent is manufactured in compliance with our ISO 9001 certified quality management system, and is in conformity with the acceptance criteria set up for the product. The Bad Merck & Co ecl select western blotting detection reagent Ecl Select Western Blotting Detection Reagent, supplied by Merck & Co, used in various techniques. GE Healthcare Amersham ECL Prime Western Blotting Detection Reagent Brand: GE Healthcare 28980926 Additional Details : Weight : 0 . 274.38 EUR - 576.30 EUR See promotional offers below This item: RPN2236 - Amersham ECL Prime Western Blotting Detection Reagent - Amersham ECL Western Blotting Prime Blocking and Blotting Reagents, GE Healthcare - Each $528.87 Usually ships within 3 to 5 weeks. Signals can be detected with CCD imager or X-ray film. ECL Molecular Weight Rainbow Markers and Amersham ECL DualVue Western Blotting Markers Optimized for CCD-based imaging, for example Amersham Imager 600 or ImageQuant LAS 500, and compatible with X-ray film such as Amersham Hyperfilm ECL Amersham ECL Select is a high sensitivity reagent As exemplified, we supply products spanning the entire spectrum of the Western blotting process. 3 One of the first commercially available chemiluminescent detection reagents Entry-level chemiluminescent detection reagent Reagent of choice for confirmatory Western blotting applications Western blotting is a valuable tool for verifying identity of recombinantly expressed proteins, as well as for identification of protein fractions to be used for further enrichment of the target . They are at least 10-fold more sensitive than colorimetric assays and produce excellent and reliable results every time. For outine and confirmatory Western blotting, high expressed proteins and tagged proteins. Amersham ECL Select is a high sensitivity reagent for chemiluminescent Western blotting detection. RPN2232 Solution A: Luminol solution, 50 ml Solution B: Peroxide solution, 50 ml sufficient for 1000 cm 2 membrane Analysis Note To view the Certificate of Analysis for this product, please visit www.cytiva.com. Availability: Out of Stock, ships in 4 to 6 Weeks. Amersham ECL Prime is a highly sensitive Western blotting detection reagent characterised by stable signal emission, allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. . Amersham ECL . Manufacturer: Cytiva RPN2134 View more versions of this product Catalog No. Amersham ECL Select is a high sensitivity reagent for chemiluminescent Western blotting detection. Western blotting detection reagents, Amersham ECL Supplier: Cytiva Amersham ECL Western Blotting System is designed for detection of mouse and rabbit primary antibodies on Western blots using HRP-conjugated secondary antibodies and ECL reagents For routine and confirmatory Western blotting applications 132.88 - 406.98 Specifications View More Specs Products 4 Product Type: Amersham ECL Prime Western Blotting Detection Reagent: Sufficient For: 1000cm 2 membrane: Kit Contents: Solution A (Luminol solution, 50mL) and Solution B (Peroxide . High signal intensity makes Amersham ECL Select suitable for the most demanding Western blotting applications, allowing for detection of minute protein quantities. The solutions should be equilibrated to at room temperature and then mixed in equal volumes directly before use. 217.26 - 449.82 . Amersham ECL Select detection reagent is designed to provide very high light output. Amersham ECL Prime is a highly sensitive reagent, characterized by stable signal emission, allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. Membranes can be reprobed up to 10 times and still provide quantifiable linear response in the range 0.2-2 OD units using pre-flashed Hyperfilm ECL. 5 3. Signals can be detected with CCD imager or X-ray film. Cytiva Life Sciences Amersham ECL Western Blotting Detection Reagents are the world's first commercially available chemiluminescent detection reagents and still one of the most widely used. ECL Western Blotting Detection Reagents, in combination with Amersham Protran membranes, form a complete solution for Western blot applications. Amersham ECL Western blotting detection reagents. RPN3243 GE Healthcare Amersham ECL start Western Blotting Detection Reagent Amersham ECL start Western Blotting Detection Reagent, 200mL Each of 1 Rating Required Select Rating 1 star (worst) 2 stars 3 stars (average) 4 stars 5 stars (best) SuperSignal West Atto provided the best signal-to-noise ratios across all protein loads. Ideal for confirmatory Western blotting applications using chemiluminescence detection At least 10-fold more sensitive than colorimetric assays Detectable on Amersham Hyperfilm ECL or with a CCD imager such as ImageQuant LAS 500 . The following components are included in the Amersham ECL Prime detection reagent kit. A comparison is made with Amersham ECL Plus reagent over the same time course. The high signal intensity can fully utilize the advantages of CCD camera based imaging equipment and makes Amersham ECL start optimal for detection with ImageQuant LAS 500 and Amersham Imager 600 from GE Healthcare. Key benefits: High intensity of the emitted signal means that Amersham ECL Prime is suitable for working with low abundance proteins Long lasting, stable signal > 3 hours Several ECL western blot kits are available for a wide range of target sensitivities. Signals can be detected with CCD camera or X-ray film. Chemiluminescence detection reagent for detection of high to medium abundant proteins. This makes Amersham ECL Select suitable for the most demanding Western blots regarding sensitivity requirements. Amersham ECL Western blotting detection reagent 400 ml for 4000 cm2 membrane 45-000-875 ECL And ECL Plus Western Blotting Detection Reagents From GE Healthcare (formerly Amersham Biosciences) The Good Strong, bright bands with both reagents. Amersham ECL Prime detection reagent is designed to provide long signal duration (24 hours) and high sensitivity (picogram levels). Specifications. Blots probed with mouse or rabbit primary antibodies are incubated with HRP-conjugated secondary antibodies. Amersham ECL Western Blotting Detection Reagents use enhanced luminol-based detection which is suitable for all routine Western blotting. Recommended for chemiluminescence and fluorescence applications where high sensitivity is needed. RPN2109 GE Healthcare Amersham ECL Western Blotting Detection Reagent Amersham ECL Western Blotting Detection Reagent Each of 1 Rating Required Select Rating 1 star (worst) 2 stars 3 stars (average) 4 stars 5 stars (best) Amersham ECL Prime is a highly sensitive chemiluminescent detection reagent and is characterized by extremely stable signal emission. *4 ECL Prime Western Blotting Detection ReagentRPN2232Anti Mouse IgG, HRP-linked Whole AntibodyNA931-100ULAnti Rabbit IgG, HRP-linked Whole AntibodyNA934-100ULECL Prime Blocking ReagentRPN418ECL DualVue . Protocols for both products are very thorough. Western blotting detection of transferrin in a 2-fold dilution series using Amersham ECL Prime (top) and Amersham ECL Plus (bottom). Each pack contains 40 g of membrane blocking agent. Bioz Stars score: 88/100, based on 1 PubMed citations. Its long lasting signals allow for multiple exposures and a convenient time window between experiment and analysis. Price: $409.40 Amersham ECL Western Blotting Detection Reagent 250 mL For 4000 cm2 membrane Specifications for this item See more product details + + This item: RPN2106 - Amersham ECL Western Blotting Detection Reagent - Amersham ECL Western Blotting Detection System, GE Healthcare - Each $409.40 Popular Categories; Antibody Search; Biomolecules; Assay Kits; . GE Healthcare has introduced Amersham ECL* Prime, a highly sensitive reagent characterized by stable signal emission up to three hours after addition of the reagent, allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. Legal Information Amersham ECL Select: The most sensitive chemiluminescence ECL reagent in the Amersham ECL range. Western blotting detection reagent, Amersham ECL start. Enhanced chemiluminescence (ECL) detection reagents for reliable detection of medium and high expression proteins. Ideal for confirmatory Western blotting applications using chemiluminescence detection At least 10-fold more sensitive than colorimetric assays Detectable on Amersham Hyperfilm ECL or with a CCD imager such as ImageQuant LAS 500. $375.25. 45-100-100 $210.05 / Each Quantity Add to cart Description Specifications Description Amersham ECL Prime is a highly sensitive reagent, characterized by stable signal emission, allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. ECL Plus Western blotting detection reagents are manufactured for GE Healthcare by Lumigen Inc. Amersham ECL start Western blotting detection reagent is designed to provide high light output. 2-8C Description General description Amersham ECL Western Blotting Detection Reagents were the worlds first commercial chemiluminescent reagents for Western blotting and are still the most widely used. Manufacturer: Cytiva RPN2106 View more versions of this product Catalog No. Amersham ECL Prime. ECL Western Blotting Detection Reagents, in combination with Amersham Protran membranes, form a complete solution for Western blot applications. Bioz Stars score: 80/100, based on 1 PubMed citations. Cytiva Life Sciences Amersham ECL Western Blotting Detection Reagents are the world's first commercially available chemiluminescent detection reagents and still one of the most widely used. The high intensity of the emitted signals . Cytiva Lifescience Amersham ECL Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity. Components ECL Advance Solution A Solution containing Tris buffer in 3.2% (v/v) Ethanol, 50 ml. Cytiva Lifescience Amersham ECL Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity. Welcome Guest. The samples GE Healthcare RPN2235 Amersham ECL Select Western Blotting Detection Reagent Amersham ECL Select Western Blotting Detection Reagent (Each of 1) (No reviews yet) Write a Review. High sensitivity Western blotting detection of ERK in HeLa cell lysate using Amersham ECL Select. Optimized for CCD-based imaging, for example Amersham Imager 600 or ImageQuant LAS 500, and compatible with X-ray film such as Amersham Hyperfilm ECL; Amersham ECL Select is a high sensitivity reagent for chemiluminescent Western blotting detection. Cytiva Amersham ECL Western Blotting Detection Reagents Use for all routine Western blotting. For you who are familiar with Amersham ECL Plus from before, Amersham ECL Prime is the replacement product that offers the same . Amersham ECL Western blotting detection reagents WB ERK ; LOD: 20 ng; DR: 2.4 5 g 20 ng WB ERK ; LOD: 5 ng; DR: 2.4 1.2 g 5 ng WB ERK (Cy5 on FLA 9500); LOD 10 ng; DR: 3 . Cytiva Life Sciences Amersham ECL Western Blotting Detection Reagents are the world's first commercially available chemiluminescent detection reagents and still one of the most widely used. Education and research. The following components are included in the Amersham ECL Prime detection reagent kit. Western Blot Detection Reagents . Products. Membranes can be reprobed up to 10 times and still provide quantifiable linear response in the range 0.2-2 OD units using pre-flashed Hyperfilm ECL. Amersham ECL detection reagent uses enhanced luminol-based detection suitable for all routine confirmatory Western blotting experiments. Important user information 3.1 Intended use Amersham ECL Select detection reagent is intended for chemiluminescent detection in Western blotting. The high intensity light output means that highly diluted antibodies can be used, and makes detection optimal using a CCD camera (e.g., ImageQuant LAS systems from GE). Fig 4. For confirmatory Western blotting applications.At least ten-fold more sensitive than colorimetric assays.Detectable on Amersham Hyperfilm ECL or with a CCD imager, for example ImageQuant LAS 500.The world's first commercially available chemiluminescent detection reagent is still one of the most widely used. Blots were incubated with the corresponding secondary antibody conjugated to horseradish peroxidase and the signal was detected using ECL Plus detection reagent according to the manufacturer's instructions (GE Healthcare) in a Luminescent Image Analyzer LAS-1000 Plus (FUJIFILM). Cytiva Life Sciences Amersham ECL Western Blotting Detection Reagents are the world's first commercially available chemiluminescent detection reagents and still one of the most widely used. Membranes can be reprobed up to 10 times and still provide quantifiable linear response in the range 0.2-2 OD units using pre-flashed Hyperfilm ECL. Shop GE Healthcare Amersham ECL Prime Western Blotting Detection Reagent at Fishersci.se English . Also compatible with Rainbow Molecular Weight Markers and Amersham ECL DualVue Western Blotting Markers; Education and research. Sufficient for at least 20 miniblots. Long term storage of the individual reagents should be in the light tight containers in which they are provided. ECL Western Blotting Detection Reagents, in combination with Amersham Protran membranes, form a complete solution for Western blot applications. Amersham ECL Western blotting detection reagents A variety of reagents are available, with the optimal choice depending on the aim of the . ECL Plus is even brighter, which is good for low protein levels. Cytiva Life Sciences Amersham ECL Western Blotting Detection Reagents are the world's first commercially available chemiluminescent detection reagents and still one of the most widely used. 10-fold more sensitive than colorimetric assays Shop Cytiva Amersham ECL Western Blotting Detection Reagents at Fishersci.co.uk English . Amersham ECL Select Western blotting detection reagent from Cytiva. Also compatible with Rainbow Molecular Weight Markers and Amersham ECL DualVue Western Blotting Markers . ECL Western Blotting Detection Reagents, in combination with Amersham Protran membranes, form a complete solution for Western blot applications. ECL Select reagent is the most sensitive chemiluminescent Western blotting detection reagent in the ECL product family. $179.40 - $485.00 Specifications View More Specs Products 4 Description Specifications Description Detectable on Hyperfilm ECL or with CCD imager 18 Nov 2010. Amersham ECL Prime delivers a detection system that is sensitive, stable, precisely quantitative across a wide dynamic range of protein . RPN2232 GE Healthcare Amersham ECL Prime Western Blotting Detection Reagent Amersham ECL Prime Western Blotting Detection Reagent Each of 1 Rating Required Select Rating 1 star (worst) 2 stars 3 stars (average) 4 stars 5 stars (best) $363.00 - $703.00 See promotional offers below Specifications ZERO BIAS - scores, article reviews, protocol conditions and more Cytiva Amersham ECL Prime Western Blotting Detection Reagent Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity. Specifications. The light output can also be 45-001-160 $466.50 / Each of 1 Qty Check Availability Add to cart Description Stable signal enables multiple exposures and makes reagent suitable for large experimental series. CCD . Amersham ECL Western Blotting Detection Kit uses horseradish peroxidase (HRP) conjugated anti-mouse and anti-rabbit antibodies for luminol-based detection of Western blots. Cytiva Life Sciences Amersham ECL Western Blotting Detection Reagents are the world's first commercially available chemiluminescent detection reagents and still one of the most widely used. High signal intensity means that . 2-8C Description General description Amersham ECL Western Blotting Detection Reagents were the worlds first commercial chemiluminescent reagents for Western blotting and are still the most widely used. 45-000-875 $359.50 / Each of 1 Qty Check Availability Add to cart Description Stable until the expiration date printed on box when stored under recommended conditions. This component is covered by US patent numbers 5491072 and 5593845 and foreign equivalents and is sold under licence from Lumigen Inc. All third party trademarks are the property of their respective owners. Cytiva Life Sciences Amersham ECL Western Blotting Detection Reagents are the world's first commercially available chemiluminescent detection reagents and still one of the most widely used. Proven reliability: For decades, Amersham ECL has been a widely used chemiluminescent detection reagent for researchers worldwide, giving excellent and reliable results every time ECL Start Western Blotting Detection Reagent; ECL Start western blotting detection reagent has been used in western blotting. Cytiva Lifescience Amersham ECL Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity. High signal intensity makes Amersham ECL Select suitable for the most demanding Western blotting applications, allowing for detection of minute protein quantities. Manufacturer: Cytiva RPN2134 View more versions of this product Catalog No. Price from $9.99 to $1999.99 enhanced chemiluminescence western blotting detection reagents - by Bioz Stars , 2022-09 99 / 100 stars Buy from Supplier enhanced chemiluminescence prime western blotting detection reagent ( GE Healthcare ) 99 GE Healthcare enhanced chemiluminescence prime western blotting detection reagent Amersham Pharmacia Biotech Ltd ecl plus western blotting detection reagent kit Ecl Plus Western Blotting Detection Reagent Kit, supplied by Amersham Pharmacia Biotech Ltd, used in various techniques. Enhanced chemiluminescence (ECL) detection reagents for reliable detection of medium and high expression proteins. Amersham ECL Plex CyDye secondary antibodies ECL Plex Cy2-, Cy3-, and Cy5-conjugated antibodies are part of an optimized system for quantitative analysis . Use for all routine Western blotting. For Use With (Application Specifications. For use with Amersham ECL start, Amersham ECL, Amersham ECL Prime, Amersham ECL Select Western Blotting Detection Reagents as well as Amersham ECL Plex. They are at least 10-fold more sensitive than colorimetric assays and produce excellent and reliable results every time. SKU: RPN2235. Amersham ECL Prime is a highly sensitive reagent, characterized by stable signal emission, allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. Resulting blot shown in A) and integrated The output Compare this item Amersham ECL Western Blotting Detection Kit MPN: RPN2235. ZERO BIAS - scores, article reviews, protocol conditions and more Optimized for imaging with ImageQuant LAS 4000 (CCD-based imaging), Amersham Hybond P (PVDF) membranes and compatible with Amersham Hyperfilm . Amersham ECL start is a chemiluminescent detection reagent in the Amersham ECL product family that is recommended for confirmatory analysis and detection of high to medium levels of endogenous proteins. Fig 4. Other Notes Color: Detection reagent 1: colorless
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