The absorbance at 340 nm was monitored continuously using a POLARstar Omega plate reader (BMG Labtech) for 5-20 min and converted into the molar concen-tration of NADH using Beer's law, assuming "340 nm = 6220 M 1cm . We're using a Polarstar Omega plate reader and black plates. Results were normalized to the basal BRET ratio before the addition of . 40266 Email: drugdiscoverytech@invitrogen.com LanthaScreenTerbium Assay Setup Guide on the BMG LABTECH POLARstar Omega Microplate Readers Bioz Stars score: 90/100, based on 1 PubMed citations. Microplate readers HTRF Certified Readers and Setups HTRF Certified Readers and Setups Configure your microplate reader HTRF is compatible with a large range of available microplate readers. The POLARstar Omega is the fully-equipped Omega-series microplate reader, offering all the features of a FLUOstar Omega, and in addition fluorescence polarization. This upgradeable reader offers the highest flexibility for optimizing settings for all absorbance-based assays. Setup Guide on the BMGLABTECH POLARstar Omega Microplate Reader Have a question? the polarstar omega is the fully-equipped omega-series microplate reader, offering all the features of a fluostar omega, and in addition fluorescence polarization.equipped with bmg labtech's ultra-fast uv/vis spectrometer and simultaneous dual emission detection, the polarstar omega represents a great combination of performance and flexibility Software: The software interface for the PHERAstar, POLARstar Omega and all other BMG LABTECH plate readers is identical, thereby allowing users to easily migrate from plate reader to plate reader without a learning curve. Microtiter plates up to a size of 1536 wells. BMG Labtech POLARstar Omega Microplate Reader Dell Latitude i5 running Omega software ver3.0 Communication and power cables. Polarstar Omega Plate Reader, supplied by BMG Labtech, used in various techniques. BMG LABTECH POLARstar Omega. The user interface is well-designed with intuitive icons to allow a new user to get up and running with minimal training. After 24 h, the cells were treated with various inhibitor compounds and incubated for an additional 24 h. Finally, luciferase activity was measured on a POLARstar Omega plate reader (BMG LABTECH). It works fine for absorbance and . Microplate Formats Up to 384-well plates, 1536-well plates in absorbance, user-de nable . 40266 Email: drugdiscoverytech@invitrogen.com 10. cAMP Signaling Assay. Upgrade able Plate Readers. Your lab might even have one already. POLARstar Omega Platereader BMG Labtech Location: 7147 MRB3 This is a multimode platereader for absorbance, luminescence and fluorescence. The FLUOstar Omega is the ideal plate reader for life science applications. Your chosen instrument can also be upgraded at any time if you need more features or additional detection modes. Applications include Ca2+ measurements (e.g. POLARstar Omega Due to the modularity of BMG LABTECH's instruments, all, or combinations of the features below can be installed . Fluorescence was measured using a POLARstar Omega plate reader. The POLARstar Omega edition of micro-plate readers able to analyze multiple . FLUOstar OMEGA is a development of the popular FLUOstar OPTIMA microplate reader and has utilized and improved both the hardware and software. Ca2+ flux with Fura-2, Indo-1, FLUO-3/4) Enzyme activity FRET/BRET assays Measurements were performed in 96 well plates (200 l volume) in a POLARstar omega plate reader (BMG Labtech). 2300 for 665 and 620 : This reader only allows high performance HTRF measurement when assays are run in WHITE plates. To calculate percentage of NETosis in each condition, the green . The POLARstar Omega is a versatile, filter-based multi-mode microplate reader with seven detection modes: fluorescence intensity (including FRET), fluorescence polarization, luminescence (including BRET), UV/vis absorbance, time-resolved fluorescence, TR-FRET, and AlphaScreen/AlphaLISA. AND SENSITIVITY FOR ALL LEVELS OF EXPERIENCE The Tristar 3 is a user-friendly and affordable filter-based multimode plate reader that offers the high-performance analysis you expect from Berthold Technologies . Plate Reader; FLUOstar Omega, Multimode, 1536 Well, BMG LabTech. Contact a supplier or the parent company directly to get a quote or to find out a price or your closest point of sale. BMG PolarStar plate reader is capable of FRET, fluorescence polarization luciferase and renilla assays, transcription and protein interaction luminescence reporter assays, protein measurement, with capacity for high-throughput and automatic reagent injection. The plate is adaptable with the following BMG LABTECH readers: Taunton, MA, USA Click to Request Price Configuration and Setup: Detection Modes: FP, FI, TRF, Luminescence,. ATPase activity assays 1 . Equipped with BMG LABTECH's ultra-fast UV/Vis spectrometer and Simultaneous Dual Emission detection, the POLARstar Omega represents a great combination of performance and flexibility for life science applications. The plate was incubated at 28 C with periodic shaking, and ThT fluorescence (excitation = 440 nm, emission = 480 nm) was measured using a POLARstar OMEGA plate reader. After a baseline read, 100 L aCSF collected from the slices was added to the cells, and the response was measured after 5 min on the POLARstar OMEGA plate reader. read more OVERVIEW ACCESSORIES DOCUMENTS ENQUIRY FORM Dedicated UV-laser excitation. Plate reader BMG Labtech POLARstar Omega for absorbance, fluorescence polarisation and luminescence, minimum wavelength 220nm, maximum wavelength 1000nm, for 96-well and 384-well plates, heated, shaking, scanning. A new window will appear. Proteins analyzed were AbpSH3 (the src homology 3 domain from Actin Binding. . Software Multi-user software package including Reader Control and MARS Data Analysis Software, FDA 21 CFR Part 11 . 96-well plates (Greiner Bio-One) at a density of 10,000 cells per well. This upgradeable reader offers the highest flexibility for optimizing settings for all absorbance-based assays. The Omega Plate Reader Series offer true flexibility to provide you with the perfect reader to fulfil your requirements: from an absorbance-only SPECTROstar or luminescence-only LUMIstar to a fully-equipped POLARstar Omega with up to seven detection modes. The plate was transferred to a POLARstar Omega plate reader (BMG Labtech) and fluorescence (excitation at 485 nm, emission at 520 nm) and O.D. Protein acetylation was assessed in mitochondria, cytoplasm and nuclei fractions. The POLARstar Omega is the fully-equipped Omega-series microplate reader, offering all the features of a FLUOstar Omega, and in addition fluorescence. Fluorescently-labelled RNA substrates and HBV Cp aliquots were titrated into the 96-well plate using a Biomek 4000 automated liquid handler robot (Beckmann Coulter). The POLARstar Omega is the fully-equipped Omega-series microplate reader, offering all the features of a FLUOstar Omega, and in addition fluorescence polarization. The FLUOstar Omega is a multi-mode microplate reader with six detection modes. Total DNA (100% DNA) present in these neutrophils was determined by the fluorescence values of the cells lysed with 0.5% (v/v) Triton-X-100. Absorbance plate reader with ultrafast UV/vis spectrometer. Filter part numbers Excitation filter: Ex TR L Emission filter: BP645-20 The Omega software supplied by BMG LABTECH should include default assay protocols and analysis templates for Valita Titer is compatible with many available plate readers and brands. Equipped with BMG LABTECH's ultra-fast UV/Vis spectrometer and Simultaneous Dual Emission detection, the POLARstar Omega represents a great combination of performance and flexibility for life science applications Western blot of total protein acetylation in mutant mtND3 (B) and mutant NDUFAF6 (C) fibroblasts. FLUOstar Omega Multimode Microplate reader with 6-detection modes and a built-in UV/Vis spectrometer. The viability of dermal fibroblasts was evaluated by incubating cells for 6 hours with the Alamar Blue Cell Viability Reagent (Thermo Fisher Scientific), and the enzymatic . It's one of the older Polarstars, probably 10-15 years old. 40266 Email: drugdiscoverytech@invitrogen.com 3. The POLARstar Omega is a versatile, filter-based multi-mode microplate reader with six detection modes: fluorescence intensity (including FRET), fluorescence polarization, luminescence (including BRET), UV/Vis absorbance, time-resolved fluorescence (including TR-FRET), and AlphaScreen /AlphaLISA . When plate reader When plate reader Figure 2: Left . The SPECTROstar Omega is a dedicated absorbance microplate reader that can capture a full absorbance spectrum (220 - 1000 nm) in less than 1 second per well - no scanning needed. Features Two matched PMTs for Simultaneous Dual Emission The software which controls the instrument is OMEGA (version 5.10 R2) and the data analysis software is MARS (version 3.02 R2). The FLUOstar Omega is a modular multi-mode microplate reader platform for flexible assay development and cellular analysis that allows versatility in choice of assay read out and detection mode. As controls, rate measurements were Having Simultaneous Dual Emission (SDE) detection, it can capture two emission wavelengths at once, like the perpendicular and parallel signals in FP. Measurements in up to 384-well plates, 1536-wells in absorbance and scans up to 900 data points/well. The unit can be configured with on-board 'smart' injectors and has a temperature controlled incubation chamber with an extended range to 60 C option. Equipped with BMG LABTECH's ultra-fast UV/vis spectrometer and Simultaneous Dual Emission detection, the POLARstar Omega represents a great combination of performance and flexibility for life science applications. The FLUOstar Omega is a multimode microplate reader perfect for life science research laboratories. The POLARstar Omega is a versatile, filter-based multi-mode microplate reader with seven detection modes: fluorescence intensity . The POLARstar Omega is the fully-equipped Omega-series microplate reader, offering all the features of a FLUOstar Omega, and in addition fluorescence polarization. Setup Guide on the BMGLABTECH POLARstar Omega Microplate Readers Have a question? In addition it has CO2 and O2 control. Supernatants were removed, cells lysed using 300 L/well DMSO, and optical density measured (OD 570) using a POLARstar Omega plate reader (BMG Labtech, Ortenberg, Germany). At this point, a new screen will open (below). Direct Reprogramming Neurons were generated from mutant NDUFAF6 and control fibroblasts by direct neuronal reprogramming as previously described by Drouin-Ouellet et al. Powers up, initialises, tray ejects and retracts smoothly, performs a read and PC loads BMG software, otherwise untested by us - IMPORTANT: TRANSPORT LOCK IS ON and must be disengaged before next power on (see photo). [28, 29]. ValitaTiter plate readers configuration. The POLARstar Omega is a versatile, filter-based multi-mode microplate reader with seven detection modes: fluorescence intensity (including FRET), fluorescence polarization, luminescence (including BRET), UV/vis absorbance, time-resolved fluorescence, TR-FRET, and AlphaScreen/AlphaLISA. Absorbance plate reader with ultrafast UV/vis spectrometer. backed by german engineering and technology, the polarstar omega is a versatile, automated microplate reader offering the following main detection modes: ultra-fast uv/vis absorbance spectra fluorescence intensity (fi), including fret fluorescence polarization/anisotropy (fp) applications time-resolved fluorescence (trf) time-resolved fret Stock Code: 415-102-AFL Wolf special offer - Plate reader BMG Labtech FLUOstar Omega for absorbance, fluorescence and luminescence, minimum wavelength 240nm, maximum wavelength 740nm, for 96-well and 384-well plates, heated, shaking. The key requirement for the plate reader is that it must have a Fluoresence Polarisation (FP) detector and optical filters to measure FITC (Excitation max = 490, Emission max = 525). COS-7 cells (American Type Culture Collection) were cultured in DMEM with 10% fetal bovine serum and incubated at 37C in a humidified 5% CO 2 Bailey and Hay, 2006 . https://www.bioz.com/result/polarstar omega microplate reader/product/BMG Labtech Average 86 stars, based on 40 article reviews Price from $9.99 to $1999.99 polarstar omega microplate reader - by Bioz Stars , 2022-09 86 / 100 stars Images 1) Product Images from "In vitro profiling of orphan G protein coupled receptor (GPCR) constitutive activity" Equipped with BMG LABTECH's ultra-fast UV/Vis spectrometer and Simultaneous Dual Emission detection, the POLARstar Omega represents a great combination of performance and flexibility for life science applications. The excitation/emission is 490/520. Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 Select option 3, ext. Built-in "smart" injectors dispense reagents and initiate kinetic reactions. Using this feature of the POLARstar Omega plate reader, we have demonstrated the sensitivity of the Pierce Luciferase Dual Reporter Assays over at least a 100,000-fold luciferase concentration range after normalization to a control reporter, typically red firefly luciferase. The BMG LABTECH PHERAstar and POLARstar Omega plate readers are superbly designed instruments with a shared, simple user interface. Omega PolarStar Plate Reader. Setup Guide on the BMGLABTECH POLARstar Omega Microplate Reader Have a question? ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/polarstar omega plate reader/product/BMG Labtech Average 90 stars, based on 1 article reviews Anisotropy measurements were performed in a 96-well microplate using a microplate reader (plate reader - POLARstar Omega, BMG Labtech, plate - Greiner Bio-One, product no. BMG POLARstar Omega Plate Reader + Dell Optiplex 9020 PC (Windows 7) & BMG Software. The SPECTROstar Omegais an absorbance microplate reader that can be upgraded to include all of the above mentioned modes. All Omega instruments achieve high-performance measurement data in a wide range of wavelengths. SERPINB14 (ovalbumin), a non-chaperone clade B serpin that shares high sequence homology with PAI-2 was used as a control in these experiments. Installation and training required to be purchased as an accessory. 600 measured for 6 h at 5-min intervals at 30C, shaking at 225 r.p.m. The versatile optical system allows easy switching from top to bottom optic. Fluorescence was measured using a POLARstar Omega plate reader (BMG Labtech, Offenburg, Germany). Features Two matched PMTs for Simultaneous Dual Emission The POLARstar Omega edition of micro-plate readers able to analyze multiple detection modes including fluorescence polarization . Find out all of the information about the BMG LABTECH - The Microplate Reader Company product: fluorescence microplate reader POLARstar Omega. The SPECTROstar Omega is a dedicated absorbance microplate reader that can capture a full absorbance spectrum (220 - 1000 nm) in less than 1 second per well - no scanning needed. Kinetic constants were calculated from binding curves using a 4 parameter logarithmic binding equation using the program PRISM (GraphPad). The reaction was stopped using 100 L of 1 M HCl and absorbance was read at 450 nm using POLARstar OMEGA plate reader. In addition, BMG LABTECH's proprietary . Immunofluorescence and confocal microscopy Measures weak fluorescence signals with high accuracy. BMG Labtech POLARstar Galaxy USED Manufacturer: BMG Labtech Microplate Readers: BMG: POLARstar Galaxy The Polarstar is a fully automated microplate based multi-detection reader which incorporates Five different measurement principles: Fluorescence intensity, Fluorescence . The Omega Plate Reader Series offers true flexibility to provide you with the perfect reader to fulfill your requirements: from an absorbance-only SPECTROstar or luminescence-only LUMIstar to a fully-equipped FLUOstar Omega. Features Two matched PMTs for Simultaneous Dual Emission 655900). The PHERAstar FS is an advanced HTS plate reader designed for all HTRF assays and features the following: All microplate formats up to 3456-well. Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 Select option 3, ext. 2.11. FP technology is becoming more commonplace in . . Like all readers in the Omega range the POLARstar Omega benefits from the unique UV/Vis spectrometer providing spectral data acquisition quicker than any traditional scanning technology. Temperature control up to 65C. A POLARstar OMEGA plate reader (BMG Labtech) was used to measure luminescence signals at 460 20 nm and 528 10 nm at 28C every 5 s and BRET calculated as the ratio between the emission intensity at 528 10 nm divided by the emission intensity at 460 20 nm. The POLARstar Omega is the fully-equipped Omega-series microplate reader, offering all the features of a FLUOstar Omega, and in addition fluorescence polarization. Size exclusion chromatography Western Blot The Evaluation Plate from BMG LABTECH has been designed specifically for the validation of the optical functionality of the company's microplate readers for the purposes of quality control and/or qualification (IQ/OQ) in environments that conform to GLP compliance. Having multiple detection modes (fluorescence polarization, fluorescence intensity, luminescence, and full spectrum UV-tis absorbance) as well as optional modes (time-resolved fluorescence and AlphaScreen . Place your plate in the reader, and select a well to use for adjusting gain. Measurements in up to 384-well plates; Top and bottom reading; Incubation up to 45 or 65 C; Well scanning with up to 900 data points per well; Equipped with BMG LABTECH's ultra-fast UV/vis spectrometer and Simultaneous Dual Emission detection, the POLARstar Omega represents a great combination of performance and flexibility for life science applications. Top and bottom reading. Capable of measuring all of the leading microplate based assays, one of its most unique features is its CCD-based spectrometer. The POLARstar Omega is a multifunction microplate reader for life science laboratories that want to obtain great results in fluorescence polarization (FP) and other advanced assays. To normalize the data, cells . If not, our scientific team will help you choose the instrument that best suits your needs and budget. Figure 3. It utilizes an ultra-fast UV/vis spectrometer or filters for absorbance as well as highly sensitive filters for all other detection modes. Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 Select option 3, ext. The POLARstar Omega is a versatile, filter-based multi-mode microplate reader with seven detection modes: fluorescence intensity (including FRET), fluorescence polarization, luminescence (including BRET), UV/vis absorbance, time-resolved fluorescence, TR-FRET, and AlphaScreen/AlphaLISA. All experiments were performed in triplicate. POLARstar Omega microplate reader from BMG LABTECH Full experimental details can be read in detail in Wang et al. Installation and training required to be. Choose the right microplate reader for your research. BMG LABTECH FLUOstar / POLARstar / LUMIstar OPTIMA Operating Manual 2009-11-27 0413B0001J 3/23 The FLUOstar OPTIMA is a multifunctional microplate reader that can perform a wide variety of applications for fluorescence intensity, time-resolved fluorescence, absorbance and luminescence.
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